TY - JOUR AV - none ID - scholars13203 TI - Biorefinery of Chlorella sorokiniana using ultra sonication assisted liquid triphasic flotation system KW - Algae; Flotation; Liquids; Microorganisms; Proteins; Refining; Sulfur compounds KW - Ammonium sulphate concentration; Biorefineries; Chlorella sorokiniana; Lipid recoveries; Micro-algae; Protein recovery; Tri-Phasic; Ultra-sonication KW - Bioconversion KW - alcohol; ammonium sulfate; lipid KW - alcohol; ammonium sulfate; biomass; concentration (composition); extraction method; microalga; protein; recycling; ultrasonics KW - Article; biomass; Chlorella sorokiniana; concentration (parameter); flotation; flow rate; microalga; nonhuman; priority journal; protein analysis; protein isolation; ultrasound; Chlorella; microalga; recycling; ultrasound KW - Chlorella sorokiniana KW - Biomass; Chlorella; Microalgae; Recycling; Sonication N2 - The aim of this work was to study the ultrasonication-assisted Liquid Tri-phasic Flotation (LTF) System to obtain lipid and protein from microalgae Chlorella sorokiniana in a single step as a novel process. In the current study, biorefinery of Chlorella sorokiniana was performed using LTF system in a single step. The highest protein recovery of 97.43 ± 1.67 and lipid recovery of 69.50 ± 0.54 were obtained. The corresponding parameters were microalgae biomass loading of 0.5 w/v, ammonium sulphate concentration of 40 w/v, volume ratio of 1:1.5 (salt:alcohol), ultrasonication pulse mode of 20 s ON/20 s OFF at 20 amplitude for 5 mins, flotation air flowrate of 100 mL/min. Additionally, recycling of alcohol phase to study the circular nature of proposed biorefinery was investigated. The proposed LTF system for extraction of proteins and lipid reduces the number of operation units required in this biorefinery approach. © 2020 Elsevier Ltd N1 - cited By 18 PB - Elsevier Ltd SN - 09608524 Y1 - 2020/// VL - 303 A1 - Koyande, A.K. A1 - Chew, K.W. A1 - Lim, J.-W. A1 - Lam, M.-K. A1 - Ho, Y.-C. A1 - Show, P.-L. JF - Bioresource Technology UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078999051&doi=10.1016%2fj.biortech.2020.122931&partnerID=40&md5=995632c41eaf34b9ce5fcc1ea0b708a4 ER -