%L scholars12938
%P 101-106
%O cited By 7
%D 2020
%J Journal of Advanced Pharmaceutical Technology and Research
%I Wolters Kluwer Medknow Publications
%R 10.4103/japtr.JAPTR₂₆₂₀
%T Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA
%X Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluronan is a versatile bio-polymer and ligand of the receptor (CD44) on cancer cells. The MCF-7 and HT-29 cancer cell lines treated with hyaluronic acid-paclitaxel (HA-PTX) showed the distinguishing morphological features of apoptosis. Flow cytometric analysis showed that HA-PTX induces apoptosis as a significant mode of cell death. The activation level of tumor suppressor protein (p53) increased after PTX treatment in MCF-7, but no changes observed in HT-29 might be due to hereditary mutations. The lack of suppression in AKT and Rho A protein suggest the use of possible inhibitors in future studies which might could play a role in increasing the sensitivity of drug towards mutated cells line and reducing the possibilities for cancer cell survival, migration, and metastasis. © 2020 Journal of Advanced Pharmaceutical Technology & Research | Published by Wolters Kluwer - Medknow.
%K adenosine diphosphate; adenosine triphosphate; hyaluronic acid; nanoparticle; paclitaxel; protein kinase B; protein p53; RhoA guanine nucleotide binding protein, apoptosis; Article; breast cancer cell line; cancer cell; cell migration; cell survival; colorectal cancer cell line; concentration response; controlled study; flow cytometry; human; human cell; metastasis; priority journal
%N 3
%A G.-E.-S. Chaudhry
%A A. Akim
%A M. Zafar
%A M. Abdullah
%A Y. Sung
%A T. Muhammad
%V 11