relation: https://khub.utp.edu.my/scholars/12938/ title: Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA creator: Chaudhry, G.-E.-S. creator: Akim, A. creator: Zafar, M. creator: Abdullah, M. creator: Sung, Y. creator: Muhammad, T. description: Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluronan is a versatile bio-polymer and ligand of the receptor (CD44) on cancer cells. The MCF-7 and HT-29 cancer cell lines treated with hyaluronic acid-paclitaxel (HA-PTX) showed the distinguishing morphological features of apoptosis. Flow cytometric analysis showed that HA-PTX induces apoptosis as a significant mode of cell death. The activation level of tumor suppressor protein (p53) increased after PTX treatment in MCF-7, but no changes observed in HT-29 might be due to hereditary mutations. The lack of suppression in AKT and Rho A protein suggest the use of possible inhibitors in future studies which might could play a role in increasing the sensitivity of drug towards mutated cells line and reducing the possibilities for cancer cell survival, migration, and metastasis. © 2020 Journal of Advanced Pharmaceutical Technology & Research | Published by Wolters Kluwer - Medknow. publisher: Wolters Kluwer Medknow Publications date: 2020 type: Article type: PeerReviewed identifier: Chaudhry, G.-E.-S. and Akim, A. and Zafar, M. and Abdullah, M. and Sung, Y. and Muhammad, T. (2020) Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA. Journal of Advanced Pharmaceutical Technology and Research, 11 (3). pp. 101-106. ISSN 22314040 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089304908&doi=10.4103%2fjaptr.JAPTR_26_20&partnerID=40&md5=10ace7e6abc8274eed54aae901a62d56 relation: 10.4103/japtr.JAPTR₂₆₂₀ identifier: 10.4103/japtr.JAPTR₂₆₂₀