@article{scholars12938, note = {cited By 7}, pages = {101--106}, year = {2020}, journal = {Journal of Advanced Pharmaceutical Technology and Research}, title = {Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA}, number = {3}, doi = {10.4103/japtr.JAPTR{$_2$}{$_6$}{$_2$}{$_0$}}, volume = {11}, publisher = {Wolters Kluwer Medknow Publications}, issn = {22314040}, author = {Chaudhry, G.-E.-S. and Akim, A. and Zafar, M. and Abdullah, M. and Sung, Y. and Muhammad, T.}, abstract = {Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluronan is a versatile bio-polymer and ligand of the receptor (CD44) on cancer cells. The MCF-7 and HT-29 cancer cell lines treated with hyaluronic acid-paclitaxel (HA-PTX) showed the distinguishing morphological features of apoptosis. Flow cytometric analysis showed that HA-PTX induces apoptosis as a significant mode of cell death. The activation level of tumor suppressor protein (p53) increased after PTX treatment in MCF-7, but no changes observed in HT-29 might be due to hereditary mutations. The lack of suppression in AKT and Rho A protein suggest the use of possible inhibitors in future studies which might could play a role in increasing the sensitivity of drug towards mutated cells line and reducing the possibilities for cancer cell survival, migration, and metastasis. {\^A}{\copyright} 2020 Journal of Advanced Pharmaceutical Technology \& Research {\ensuremath{|}} Published by Wolters Kluwer - Medknow.}, keywords = {adenosine diphosphate; adenosine triphosphate; hyaluronic acid; nanoparticle; paclitaxel; protein kinase B; protein p53; RhoA guanine nucleotide binding protein, apoptosis; Article; breast cancer cell line; cancer cell; cell migration; cell survival; colorectal cancer cell line; concentration response; controlled study; flow cytometry; human; human cell; metastasis; priority journal}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85089304908&doi=10.4103\%2fjaptr.JAPTR\%5f26\%5f20&partnerID=40&md5=10ace7e6abc8274eed54aae901a62d56} }