eprintid: 12694
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/01/26/94
datestamp: 2023-11-10 03:27:15
lastmod: 2023-11-10 03:27:15
status_changed: 2023-11-10 01:49:18
type: article
metadata_visibility: show
creators_name: Semail, N.-F.
creators_name: Abdul Keyon, A.S.
creators_name: Saad, B.
creators_name: Noordin, S.S.
creators_name: Nik Mohamed Kamal, N.N.S.
creators_name: Mohamad Zain, N.N.
creators_name: Azizi, J.
creators_name: Kamaruzaman, S.
creators_name: Yahaya, N.
title: Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review
ispublished: pub
keywords: Biomolecules, Analytical method development; Analytical methodology; Analytical separations; Antineoplastic agents; Back ground electrolyte; Detection sensitivity; Electrophoretic methods; Fluorinated pyrimidines, Metabolites, floxuridine; fluorouracil; fluorouridine; molecularly imprinted polymer, Article; cost effectiveness analysis; drug determination; electrophoresis; gas chromatography; high performance liquid chromatography; human; nonhuman; solid phase microextraction; tandem mass spectrometry; ultra performance liquid chromatography
note: cited By 8
abstract: 5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. © 2020 Taylor & Francis Group, LLC.
date: 2020
publisher: Bellwether Publishing, Ltd.
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087640894&doi=10.1080%2f10826076.2020.1781654&partnerID=40&md5=0411094d07b6b56c381025493618f166
id_number: 10.1080/10826076.2020.1781654
full_text_status: none
publication: Journal of Liquid Chromatography and Related Technologies
volume: 43
number: 15-16
pagerange: 562-579
refereed: TRUE
issn: 10826076
citation:   Semail, N.-F. and Abdul Keyon, A.S. and Saad, B. and Noordin, S.S. and Nik Mohamed Kamal, N.N.S. and Mohamad Zain, N.N. and Azizi, J. and Kamaruzaman, S. and Yahaya, N.  (2020) Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review.  Journal of Liquid Chromatography and Related Technologies, 43 (15-16).  pp. 562-579.  ISSN 10826076