eprintid: 12246
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/01/22/46
datestamp: 2023-11-10 03:26:47
lastmod: 2023-11-10 03:26:47
status_changed: 2023-11-10 01:17:13
type: article
metadata_visibility: show
creators_name: Wang, C.
creators_name: Lakshmipriya, T.
creators_name: Gopinath, S.C.B.
title: Amine-Aldehyde Chemical Conjugation on a Potassium Hydroxide-Treated Polystyrene ELISA Surface for Nanosensing an HIV-p24 Antigen
ispublished: pub
keywords: Aldehydes; Antigens; Chemical analysis; Diagnosis; Enzymes; Molecular orientation; Molecules; Polystyrenes; Potassium hydroxide; Surface chemistry; Viruses, 3-(aminopropyl)triethoxysilane; Disease surveillance; Efficient detection; Enzyme linked immunosorbent assay; Heterogeneous tests; Human immunodeficiency virus; Maximum sensitivity; Surface chemical functionalization, Diseases
note: cited By 16
abstract: The enzyme-linked immunosorbent assay (ELISA) has been widely used for disease surveillance and drug screening due to its relatively higher accuracy and sensitivity. Fine-tuning the ELISA is mandatory to elevate the specific detection of biomolecules at a lower abundance. Towards this end, higher molecular capture on the polystyrene (PS) ELISA surface is crucial for efficient detection, and it could be attained by immobilizing the molecules in the correct orientation. It is highly challenging to immobilize protein molecules in a well-aligned manner on an ELISA surface due to charge variations. We employed a 3-(aminopropyl) triethoxysilane (APTES)- and glutaraldehyde (GLU)-coupled PS surface chemical strategy to demonstrate the high performance with ELISA. A potassium hydroxide treatment followed by an equal ratio of 1 APTES and GLU attachment was found to be optimal, and a longer incubation with GLU favored maximum sensitivity. p24 is a vital early secreting antigen for diagnosing human immunodeficiency virus (HIV), and it has been used for efficient detection with the above chemistry. Three different procedures were followed, and they led to the improved detection of the HIV-p24 antigen at 1 nM, which is a 30-fold higher level compared to a conventional ELISA surface. The surface chemical functionalization shown here also displays a higher specificity with human serum and HIV-TAT. The above approach with the designed surface chemistry could also be recommended for disease diagnosis on other sensing surfaces involving the interaction of the probe and the analyte in heterogeneous test samples. © 2019, The Author(s).
date: 2019
publisher: Springer New York LLC
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060872603&doi=10.1186%2fs11671-018-2848-z&partnerID=40&md5=8322e7d45926447450f9c53e0d1ceb1a
id_number: 10.1186/s11671-018-2848-z
full_text_status: none
publication: Nanoscale Research Letters
volume: 14
refereed: TRUE
issn: 19317573
citation:   Wang, C. and Lakshmipriya, T. and Gopinath, S.C.B.  (2019) Amine-Aldehyde Chemical Conjugation on a Potassium Hydroxide-Treated Polystyrene ELISA Surface for Nanosensing an HIV-p24 Antigen.  Nanoscale Research Letters, 14.   ISSN 19317573