eprintid: 12139
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/01/21/39
datestamp: 2023-11-10 03:26:41
lastmod: 2023-11-10 03:26:41
status_changed: 2023-11-10 01:16:58
type: article
metadata_visibility: show
creators_name: Alkhatib, M.F.R.
creators_name: Bahrudin, N.A.
creators_name: Salleh, H.M.
creators_name: Nasef, M.M.E.
creators_name: Ting, T.M.
title: Lipase immobilization on fibers grafted with polyglycidyl methachrylate
ispublished: pub
note: cited By 6
abstract: Lipase enzyme originated from wheat germ was immobilized on nylon -6- grafted with polyglycidyl methachrylate (PGMA). The immobilization of enzyme experiments were designed and studied using face centred central composite design (FCCCD) under response surface methodology (RSM). Prior to immobilization, the polymer was activated with diethyl amine/ethanol to introduce an amine functional group to facilitate covalent bonding with the enzyme. The immobilized and free enzymes were characterized for effect of temperature and pH on enzyme activity, stability, storage and reusability as well as kinetics studies. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. The optimum temperatures and pH for immobilized and free enzymes were 45 °C and 35 °C, and 8 and 7, respectively. The immobilized enzyme showed higher stability compared to free enzyme. The immobilized enzyme retained 18 of its activity after being recycled 8 times. In a storage stability test, immobilized lipase was able to retain 70 of its activity after being stored for 30 days, while free enzyme activity dropped to 15  after 20 days of storage. © 2019, International Islamic University Malaysia-IIUM.
date: 2019
publisher: International Islamic University Malaysia-IIUM
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067368060&doi=10.31436%2fiiumej.v20i1.1002&partnerID=40&md5=342e6f1a2c048b10a29fe62e84b8e474
id_number: 10.31436/iiumej.v20i1.1002
full_text_status: none
publication: IIUM Engineering Journal
volume: 20
number: 1
pagerange: 12-23
refereed: TRUE
issn: 1511788X
citation:   Alkhatib, M.F.R. and Bahrudin, N.A. and Salleh, H.M. and Nasef, M.M.E. and Ting, T.M.  (2019) Lipase immobilization on fibers grafted with polyglycidyl methachrylate.  IIUM Engineering Journal, 20 (1).  pp. 12-23.  ISSN 1511788X