@article{scholars11692,
            year = {2019},
         journal = {Journal of Molecular Liquids},
       publisher = {Elsevier B.V.},
           pages = {226--233},
            note = {cited By 58},
          volume = {278},
             doi = {10.1016/j.molliq.2019.01.063},
           title = {Ionic liquids with methotrexate moieties as a potential anticancer prodrug: Synthesis, characterization and solubility evaluation},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060078342&doi=10.1016\%2fj.molliq.2019.01.063&partnerID=40&md5=f0ae32f5c42f31ba359006da8d75e5ef},
        keywords = {Biocompatibility; Body fluids; Cell culture; Cytotoxicity; Drug products; Esters; Ionic liquids; Lanthanum compounds; Mammals; Salts; Sodium; Solubility; Thermogravimetric analysis, Active pharmaceutical ingredients; Mammalian cell lines; Methotrexate; Methotrexate (MTX); Orders of magnitude; Phosphate-buffered salines; Simulated body fluids; Technological utility, Drug delivery},
        abstract = {The technological utility of active pharmaceutical ingredients (APIs) is enormously improved when they are converted into ionic liquids (ILs). API-ILs possess better aqueous solubility and thermal stability than that of solid-state salt or crystalline drugs. However, many such API-ILs are not biocompatible or biodegradable. In the current study, we synthesized a series of IL-APIs using methotrexate (MTX), a potential anticancer prodrug, and biocompatible IL-forming cations (choline and amino acid esters). The MTX-IL moieties were characterized through 1 H NMR, FTIR, p-XRD, DSC and thermogravimetric analysis. The solubility of the MTX-ILs was evaluated in simulated body fluids (phosphate-buffered saline, simulated gastric, and simulated intestinal fluids). An assessment of the in vitro antitumor activity of the MTX-ILs in a mammalian cell line (HeLa cells) was used to evaluate their cytotoxicity. The MTX-ILs showed aqueous solubility at least 5000 times higher than that of free MTX and two orders of magnitude higher compared with that of a sodium salt of MTX in both water and simulated body fluids. Importantly, a proline ethyl ester MTX prodrug showed similar solubility as the MTX sodium salt but it provided improved in vitro antitumor activity. These results clearly suggest that the newly synthesized API-ILs represent promising potential drug formulations. {\^A}{\copyright} 2019 Elsevier B.V.},
          author = {Moshikur, R. M. and Chowdhury, M. R. and Wakabayashi, R. and Tahara, Y. and Moniruzzaman, M. and Goto, M.},
            issn = {01677322}
}