relation: https://khub.utp.edu.my/scholars/1169/ title: Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment creator: Zahed, M.A. creator: Aziz, H.A. creator: Mohajeri, L. creator: Mohajeri, S. creator: Kutty, S.R.M. creator: Isa, M.H. description: Response surface methodology (RSM) was employed to optimize nitrogen and phosphorus concentrations for removal of n-alkanes from crude oil contaminated seawater samples in batch reactors. Erlenmeyer flasks were used as bioreactors; each containing 250. mL dispersed crude oil contaminated seawater, indigenous acclimatized microorganism and different amounts of nitrogen and phosphorus based on central composite design (CCD). Samples were extracted and analyzed according to US-EPA protocols using a gas chromatograph. During 28 days of bioremediation, a maximum of 95 total aliphatic hydrocarbons removal was observed. The obtained Model F-value of 267.73 and probability F<0.0001 implied the model was significant. Numerical condition optimization via a quadratic model, predicted 98 n-alkanes removal for a 20-day laboratory bioremediation trial using nitrogen and phosphorus concentrations of 13.62 and 1.39. mg/L, respectively. In actual experiments, 95 removal was observed under these conditions. © 2010 Elsevier B.V. date: 2010 type: Article type: PeerReviewed identifier: Zahed, M.A. and Aziz, H.A. and Mohajeri, L. and Mohajeri, S. and Kutty, S.R.M. and Isa, M.H. (2010) Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment. Journal of Hazardous Materials, 184 (1-3). pp. 350-356. ISSN 03043894 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957878180&doi=10.1016%2fj.jhazmat.2010.08.043&partnerID=40&md5=61c86bbf1a2146c7740ea2307b5cf919 relation: 10.1016/j.jhazmat.2010.08.043 identifier: 10.1016/j.jhazmat.2010.08.043