@article{scholars1169,
         journal = {Journal of Hazardous Materials},
           pages = {350--356},
            year = {2010},
           title = {Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment},
          number = {1-3},
            note = {cited By 20},
          volume = {184},
             doi = {10.1016/j.jhazmat.2010.08.043},
            issn = {03043894},
          author = {Zahed, M. A. and Aziz, H. A. and Mohajeri, L. and Mohajeri, S. and Kutty, S. R. M. and Isa, M. H.},
        keywords = {Actual experiments; Aliphatic hydrocarbons; Aquatic environments; Central composite designs; Contaminated seawater; Dispersed crude oil; Erlenmeyer flasks; Experimental methodology; Gas chromatographs; n-Alkanes; Numerical condition; Petroleum hydrocarbons; Phosphorus concentration; Quadratic models; Response Surface Methodology; RSM, Batch reactors; Biodegradation; Bioremediation; Biotechnology; Bottles; Degradation; Environmental Protection Agency; Extraction; Marine pollution; Microbiology; Nitrogen; Nitrogen removal; Oceanography; Optimization; Organic compounds; Paraffins; Petroleum chemistry; Phosphorus; Pollution; Probability density function; Seawater, Crude oil, alkane derivative; hydrocarbon; nitrogen; petroleum; phosphorus; sea water, alkane; bioreactor; bioremediation; crude oil; experimental study; gas chromatography; marine pollution; microorganism; nitrogen; oil spill; optimization; petroleum hydrocarbon; phosphorus; probability, aquatic environment; article; batch reactor; biodegradation; bioremediation; concentration (parameters); experimental study; gas chromatography; laboratory test; microorganism; response surface method; statistical analysis; water contamination, Acids; Alkanes; Chromatography, Gas; Environmental Remediation; Models, Statistical; Water Pollutants, Chemical},
             url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-77957878180&doi=10.1016\%2fj.jhazmat.2010.08.043&partnerID=40&md5=61c86bbf1a2146c7740ea2307b5cf919},
        abstract = {Response surface methodology (RSM) was employed to optimize nitrogen and phosphorus concentrations for removal of n-alkanes from crude oil contaminated seawater samples in batch reactors. Erlenmeyer flasks were used as bioreactors; each containing 250. mL dispersed crude oil contaminated seawater, indigenous acclimatized microorganism and different amounts of nitrogen and phosphorus based on central composite design (CCD). Samples were extracted and analyzed according to US-EPA protocols using a gas chromatograph. During 28 days of bioremediation, a maximum of 95 total aliphatic hydrocarbons removal was observed. The obtained Model F-value of 267.73 and probability F{\ensuremath{<}}0.0001 implied the model was significant. Numerical condition optimization via a quadratic model, predicted 98 n-alkanes removal for a 20-day laboratory bioremediation trial using nitrogen and phosphorus concentrations of 13.62 and 1.39. mg/L, respectively. In actual experiments, 95 removal was observed under these conditions. {\^A}{\copyright} 2010 Elsevier B.V.}
}