%X Biodiesel is one of the effective alternative fuels to overcome the problems associated with environmental issues and energy crisis. However, the production of biodiesel from edible oil has provoked the food versus fuel dispute. Thus, a non-edible crop, Annona muricata, is selected as the raw material to produce oil for biodiesel production. In this study, A. muricata oil was extracted with n-hexane at 70 °C for 6 h. Subsequently, the oil was transesterified with methanol and potassium hydroxide (KOH) to produce biodiesel. The significance of transesterification parameters including temperature, time, catalyst concentration, and oil-To-methanol ratio on biodiesel yield () was established through two-level factorial design. The factorial design shows that all parameters are significant. Besides, the high content of monounsaturated fatty acid (oleic acid) in the resultant biodiesel suggests that A. muricata biodiesel could display good fuel quality. This hypothesis is proven by the excellent calorific value (39.21 MJ kg-1). © The Authors, published by EDP Sciences, 2019. %K Alternative fuels; Energy policy; Hexane; Methanol; Monounsaturated fatty acids; Oils and fats; Potassium hydroxide, Biodiesel production; Catalyst concentration; Energy crisis; Environmental issues; Factorial design; Fuel quality; High-content; Two level factorial designs, Biodiesel %D 2019 %R 10.1051/e3sconf/20199001011 %O cited By 0; Conference of 7th Conference on Emerging Energy and Process Technology, CONCEPT 2018 ; Conference Date: 27 November 2018 Through 28 November 2018; Conference Code:146656 %L scholars11643 %J E3S Web of Conferences %T Production of biodiesel from Annona muricata seeds %A W.-L. Wong %A W.-H. Lim %A J. Si %A M.-K. Lam %A Y.-C. Ho %I EDP Sciences %V 90