TY  - JOUR
N1  - cited By 65
TI  - Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: A prognosticative approach for â??Royal diseaseâ??
SN  - 09565663
JF  - Biosensors and Bioelectronics
EP  - 135
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062145936&doi=10.1016%2fj.bios.2019.02.006&partnerID=40&md5=36c041c742ddf5d2b8f0884698ae0470
N2  - This article is clearly presenting the development of a biosensor for human factor IX (FIX) to diagnose the blood clotting deficiency, a so-called â??Royal diseaseâ?? using an interdigitated electrode (IDE) with the zinc oxide surface modification. Gold nano-urchins (GNUs) with 60 nm in diameter was integrated into a streptavidin-biotinylated aptamer strategy to enhance the active surface area. Two different comparative studies have been done to validate the system to be practiced in the current work holds with a higher capability for the high-performance sense. Whereby, the presence and absence of GNUs in the aptasensing system for FIX interaction were investigated using the amperometric measurement, using a linear sweep voltage of 0â??2 V at 0.01 V step voltage. The detection limit was 6 pM based on 3Ï? calculation when GNUs integrated aptamer assay was utilized for FIX detection, which shows 8 folds sensitivity enhancement comparing the condition in the absence of GNU and 50 folds higher than sensitive radio-isotope and surface plasmon resonance assays. Albeit, the surface and molecular characterizations were well demonstrated by scanning electron microscopy, atomic force microscopy, 3D nano-profilometry and further supports were rendered by UVâ??Vis spectroscopy and Enzyme-linked apta-sorbent assay (ELASA). Furthermore, the spiking experiment was done by FIX-spikes in human blood serum in order to demonstrate the stability with a higher non-fouling. © 2019 Elsevier B.V.
ID  - scholars11631
AV  - none
Y1  - 2019///
KW  - Atomic force microscopy; Gold; II-VI semiconductors; Proteins; Scanning electron microscopy; Surface plasmon resonance; Surface treatment; Zinc oxide
KW  -  Amperometry; Aptamers; ELASA; Factor ix; Gold nano
KW  -  Blood
KW  -  blood clotting factor 9; gold nanoparticle; radioisotope; zinc oxide; aptamer; blood clotting factor; blood clotting factor 9; gold; streptavidin
KW  -  Article; atomic force microscopy; biotinylation; chemical modification; electric potential; enzyme linked immunosorbent assay; hemophilia; intermethod comparison; limit of detection; process development; profilometry; prognosis; protein binding; scanning electron microscopy; sensitivity analysis; surface area; surface plasmon resonance; ultraviolet visible spectroscopy; blood clotting; chemistry; electrode; genetic procedures; genetics; human; isolation and purification
KW  -  Aptamers
KW  -  Nucleotide; Biosensing Techniques; Blood Coagulation; Blood Coagulation Factors; Electrodes; Factor IX; Gold; Humans; Limit of Detection; Prognosis; Streptavidin; Surface Plasmon Resonance
PB  - Elsevier Ltd
A1  - Letchumanan, I.
A1  - Gopinath, S.C.B.
A1  - Md Arshad, M.K.
A1  - Anbu, P.
A1  - Lakshmipriya, T.
SP  - 128
VL  - 131
ER  -