relation: https://khub.utp.edu.my/scholars/11631/
title: Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: A prognosticative approach for �Royal disease�
creator: Letchumanan, I.
creator: Gopinath, S.C.B.
creator: Md Arshad, M.K.
creator: Anbu, P.
creator: Lakshmipriya, T.
description: This article is clearly presenting the development of a biosensor for human factor IX (FIX) to diagnose the blood clotting deficiency, a so-called �Royal disease� using an interdigitated electrode (IDE) with the zinc oxide surface modification. Gold nano-urchins (GNUs) with 60 nm in diameter was integrated into a streptavidin-biotinylated aptamer strategy to enhance the active surface area. Two different comparative studies have been done to validate the system to be practiced in the current work holds with a higher capability for the high-performance sense. Whereby, the presence and absence of GNUs in the aptasensing system for FIX interaction were investigated using the amperometric measurement, using a linear sweep voltage of 0�2 V at 0.01 V step voltage. The detection limit was 6 pM based on 3� calculation when GNUs integrated aptamer assay was utilized for FIX detection, which shows 8 folds sensitivity enhancement comparing the condition in the absence of GNU and 50 folds higher than sensitive radio-isotope and surface plasmon resonance assays. Albeit, the surface and molecular characterizations were well demonstrated by scanning electron microscopy, atomic force microscopy, 3D nano-profilometry and further supports were rendered by UV�Vis spectroscopy and Enzyme-linked apta-sorbent assay (ELASA). Furthermore, the spiking experiment was done by FIX-spikes in human blood serum in order to demonstrate the stability with a higher non-fouling. © 2019 Elsevier B.V.
publisher: Elsevier Ltd
date: 2019
type: Article
type: PeerReviewed
identifier:   Letchumanan, I. and Gopinath, S.C.B. and Md Arshad, M.K. and Anbu, P. and Lakshmipriya, T.  (2019) Gold nano-urchin integrated label-free amperometric aptasensing human blood clotting factor IX: A prognosticative approach for �Royal disease�.  Biosensors and Bioelectronics, 131.  pp. 128-135.  ISSN 09565663     
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062145936&doi=10.1016%2fj.bios.2019.02.006&partnerID=40&md5=36c041c742ddf5d2b8f0884698ae0470
relation: 10.1016/j.bios.2019.02.006
identifier: 10.1016/j.bios.2019.02.006