eprintid: 11450 rev_number: 2 eprint_status: archive userid: 1 dir: disk0/00/01/14/50 datestamp: 2023-11-10 03:25:58 lastmod: 2023-11-10 03:25:58 status_changed: 2023-11-10 01:15:18 type: article metadata_visibility: show creators_name: Phwan, C.K. creators_name: Chew, K.W. creators_name: Sebayang, A.H. creators_name: Ong, H.C. creators_name: Ling, T.C. creators_name: Malek, M.A. creators_name: Ho, Y.-C. creators_name: Show, P.L. title: Effects of acids pre-treatment on the microbial fermentation process for bioethanol production from microalgae ispublished: pub keywords: Acetic acid; Bioethanol; Energy utilization; Ethanol; Fermentation; Microorganisms; pH; Process control; Sulfuric acid, Bio-ethanol production; Bio-ethanols; Carbohydrate content; Effect of acids; Micro-algae; Microbial fermentation process; Pre-treatments; Reducing sugar concentration; Reducing sugars; Sugar concentration, Microalgae, biomass; carbohydrate; chemical bonding; concentration (composition); ethanol; fermentation; microalga; microbiology note: cited By 74 abstract: Background: Microalgae are one of the promising feedstock that consists of high carbohydrate content which can be converted into bioethanol. Pre-treatment is one of the critical steps required to release fermentable sugars to be used in the microbial fermentation process. In this study, the reducing sugar concentration of Chlorella species was investigated by pre-treating the biomass with dilute sulfuric acid and acetic acid at different concentrations 1, 3, 5, 7, and 9 (v/v). Results: 3,5-Dinitrosalicylic acid (DNS) method, FTIR, and GC-FID were employed to evaluate the reducing sugar concentration, functional groups of alcohol bonds and concentration of bioethanol, respectively. The two-way ANOVA results (p < 0.05) indicated that there was a significant difference in the concentration and type of acids towards bioethanol production. The highest bioethanol yield obtained was 0.28 g ethanol/g microalgae which was found in microalgae sample pre-treated with 5 (v/v) sulfuric acid while 0.23 g ethanol/g microalgal biomass was presented in microalgae sample pre-treated with 5 (v/v) acetic acid. Conclusion: The application of acid pre-treatment on microalgae for bioethanol production will contribute to higher effectiveness and lower energy consumption compared to other pre-treatment methods. The findings from this study are essential for the commercial production of bioethanol from microalgae. © 2019 The Author(s). date: 2019 publisher: BioMed Central Ltd official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073892870&doi=10.1186%2fs13068-019-1533-5&partnerID=40&md5=75a52eb040475f62ddc15ed5e2c09c8a id_number: 10.1186/s13068-019-1533-5 full_text_status: none publication: Biotechnology for Biofuels volume: 12 number: 1 refereed: TRUE issn: 17546834 citation: Phwan, C.K. and Chew, K.W. and Sebayang, A.H. and Ong, H.C. and Ling, T.C. and Malek, M.A. and Ho, Y.-C. and Show, P.L. (2019) Effects of acids pre-treatment on the microbial fermentation process for bioethanol production from microalgae. Biotechnology for Biofuels, 12 (1). ISSN 17546834