TY - JOUR N2 - Background: Microalgae are one of the promising feedstock that consists of high carbohydrate content which can be converted into bioethanol. Pre-treatment is one of the critical steps required to release fermentable sugars to be used in the microbial fermentation process. In this study, the reducing sugar concentration of Chlorella species was investigated by pre-treating the biomass with dilute sulfuric acid and acetic acid at different concentrations 1, 3, 5, 7, and 9 (v/v). Results: 3,5-Dinitrosalicylic acid (DNS) method, FTIR, and GC-FID were employed to evaluate the reducing sugar concentration, functional groups of alcohol bonds and concentration of bioethanol, respectively. The two-way ANOVA results (p < 0.05) indicated that there was a significant difference in the concentration and type of acids towards bioethanol production. The highest bioethanol yield obtained was 0.28 g ethanol/g microalgae which was found in microalgae sample pre-treated with 5 (v/v) sulfuric acid while 0.23 g ethanol/g microalgal biomass was presented in microalgae sample pre-treated with 5 (v/v) acetic acid. Conclusion: The application of acid pre-treatment on microalgae for bioethanol production will contribute to higher effectiveness and lower energy consumption compared to other pre-treatment methods. The findings from this study are essential for the commercial production of bioethanol from microalgae. © 2019 The Author(s). IS - 1 N1 - cited By 74 KW - Acetic acid; Bioethanol; Energy utilization; Ethanol; Fermentation; Microorganisms; pH; Process control; Sulfuric acid KW - Bio-ethanol production; Bio-ethanols; Carbohydrate content; Effect of acids; Micro-algae; Microbial fermentation process; Pre-treatments; Reducing sugar concentration; Reducing sugars; Sugar concentration KW - Microalgae KW - biomass; carbohydrate; chemical bonding; concentration (composition); ethanol; fermentation; microalga; microbiology TI - Effects of acids pre-treatment on the microbial fermentation process for bioethanol production from microalgae ID - scholars11450 AV - none UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85073892870&doi=10.1186%2fs13068-019-1533-5&partnerID=40&md5=75a52eb040475f62ddc15ed5e2c09c8a A1 - Phwan, C.K. A1 - Chew, K.W. A1 - Sebayang, A.H. A1 - Ong, H.C. A1 - Ling, T.C. A1 - Malek, M.A. A1 - Ho, Y.-C. A1 - Show, P.L. JF - Biotechnology for Biofuels VL - 12 Y1 - 2019/// SN - 17546834 PB - BioMed Central Ltd ER -