%R 10.1016/j.ijbiomac.2019.05.044
%D 2019
%V 134
%T Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy
%O cited By 46
%K biotin; gold nanoparticle; protein E7; sodium chloride; streptavidin; tumor marker; virus DNA; biological marker; biotin; gold; metal nanoparticle; oncogene protein E7, Human papillomavirus type 16; protein E7; streptavidin; virus DNA, Article; controlled study; DNA sequence; Human papillomavirus type 16; limit of detection; nonhuman; uterine cervix cancer; Wart virus; chemistry; female; genetic procedures; genetics; human; metabolism; nucleotide sequence; procedures; uterine cervix tumor; virology, Base Sequence; Biomarkers; Biosensing Techniques; Biotin; DNA, Viral; Female; Gold; Humans; Limit of Detection; Metal Nanoparticles; Papillomavirus E7 Proteins; Streptavidin; Uterine Cervical Neoplasms
%L scholars11439
%P 354-360
%A Q. Lv
%A Y. Wang
%A C. Su
%A T. Lakshmipriya
%A S.C.B. Gopinath
%A K. Pandian
%A V. Perumal
%A Y. Liu
%I Elsevier B.V.
%X Human papillomavirus (HPV) is a double-standard DNA virus, as well as the source of infection to the mucous membrane. It is a sexually transmitted disease that brings the changes in the cervix cells. Oncogenes, E6 and E7 play a pivotal role in the HPV infection. Identifying these genes to detect HPV strains, especially a prevalent HPV16 strain, will bring a great impact. Among different sensing strategies for pathogens, the dielectric electrochemical biosensor shows the potential due to its higher sensitivity. In this research, HPV16-E7 DNA sequence was detected on the carbodiimidazole-modified interdigitated electrode (IDE) surface with the detection limit of 1 fM. To enhance the sensitivity, the target sequence was conjugated on gold nanoparticle (GNP) and attained detection to the level of 10 aM. This produced ~100 folds improvement in detecting HPV16-E7 gene and 4 folds increment in the current flow. The stability of HPV16-E7 DNA sequences on GNP was verified by the salt-induced GNP aggregation. The current system has shown the higher specificity by comparing against non-complementary and triple-mismatched DNA sequences of HPV16-E7. This demonstration in detecting HPV16-E7 using dielectric IDE sensing system with a higher sensitivity can be recommended for detecting a wide range of disease-causing DNA-markers. © 2019
%J International Journal of Biological Macromolecules