TY  - JOUR
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065621740&doi=10.1016%2fj.ijbiomac.2019.05.044&partnerID=40&md5=51168ea44c1e3b85e3dd99c81cb71b6d
EP  - 360
ID  - scholars11439
N2  - Human papillomavirus (HPV) is a double-standard DNA virus, as well as the source of infection to the mucous membrane. It is a sexually transmitted disease that brings the changes in the cervix cells. Oncogenes, E6 and E7 play a pivotal role in the HPV infection. Identifying these genes to detect HPV strains, especially a prevalent HPV16 strain, will bring a great impact. Among different sensing strategies for pathogens, the dielectric electrochemical biosensor shows the potential due to its higher sensitivity. In this research, HPV16-E7 DNA sequence was detected on the carbodiimidazole-modified interdigitated electrode (IDE) surface with the detection limit of 1 fM. To enhance the sensitivity, the target sequence was conjugated on gold nanoparticle (GNP) and attained detection to the level of 10 aM. This produced ~100 folds improvement in detecting HPV16-E7 gene and 4 folds increment in the current flow. The stability of HPV16-E7 DNA sequences on GNP was verified by the salt-induced GNP aggregation. The current system has shown the higher specificity by comparing against non-complementary and triple-mismatched DNA sequences of HPV16-E7. This demonstration in detecting HPV16-E7 using dielectric IDE sensing system with a higher sensitivity can be recommended for detecting a wide range of disease-causing DNA-markers. © 2019
TI  - Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy
N1  - cited By 46
JF  - International Journal of Biological Macromolecules
SN  - 01418130
VL  - 134
Y1  - 2019///
AV  - none
A1  - Lv, Q.
A1  - Wang, Y.
A1  - Su, C.
A1  - Lakshmipriya, T.
A1  - Gopinath, S.C.B.
A1  - Pandian, K.
A1  - Perumal, V.
A1  - Liu, Y.
PB  - Elsevier B.V.
SP  - 354
KW  - biotin; gold nanoparticle; protein E7; sodium chloride; streptavidin; tumor marker; virus DNA; biological marker; biotin; gold; metal nanoparticle; oncogene protein E7
KW  -  Human papillomavirus type 16; protein E7; streptavidin; virus DNA
KW  -  Article; controlled study; DNA sequence; Human papillomavirus type 16; limit of detection; nonhuman; uterine cervix cancer; Wart virus; chemistry; female; genetic procedures; genetics; human; metabolism; nucleotide sequence; procedures; uterine cervix tumor; virology
KW  -  Base Sequence; Biomarkers; Biosensing Techniques; Biotin; DNA
KW  -  Viral; Female; Gold; Humans; Limit of Detection; Metal Nanoparticles; Papillomavirus E7 Proteins; Streptavidin; Uterine Cervical Neoplasms
ER  -