relation: https://khub.utp.edu.my/scholars/11439/
title: Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy
creator: Lv, Q.
creator: Wang, Y.
creator: Su, C.
creator: Lakshmipriya, T.
creator: Gopinath, S.C.B.
creator: Pandian, K.
creator: Perumal, V.
creator: Liu, Y.
description: Human papillomavirus (HPV) is a double-standard DNA virus, as well as the source of infection to the mucous membrane. It is a sexually transmitted disease that brings the changes in the cervix cells. Oncogenes, E6 and E7 play a pivotal role in the HPV infection. Identifying these genes to detect HPV strains, especially a prevalent HPV16 strain, will bring a great impact. Among different sensing strategies for pathogens, the dielectric electrochemical biosensor shows the potential due to its higher sensitivity. In this research, HPV16-E7 DNA sequence was detected on the carbodiimidazole-modified interdigitated electrode (IDE) surface with the detection limit of 1 fM. To enhance the sensitivity, the target sequence was conjugated on gold nanoparticle (GNP) and attained detection to the level of 10 aM. This produced ~100 folds improvement in detecting HPV16-E7 gene and 4 folds increment in the current flow. The stability of HPV16-E7 DNA sequences on GNP was verified by the salt-induced GNP aggregation. The current system has shown the higher specificity by comparing against non-complementary and triple-mismatched DNA sequences of HPV16-E7. This demonstration in detecting HPV16-E7 using dielectric IDE sensing system with a higher sensitivity can be recommended for detecting a wide range of disease-causing DNA-markers. © 2019
publisher: Elsevier B.V.
date: 2019
type: Article
type: PeerReviewed
identifier:   Lv, Q. and Wang, Y. and Su, C. and Lakshmipriya, T. and Gopinath, S.C.B. and Pandian, K. and Perumal, V. and Liu, Y.  (2019) Human papilloma virus DNA-biomarker analysis for cervical cancer: Signal enhancement by gold nanoparticle-coupled tetravalent streptavidin-biotin strategy.  International Journal of Biological Macromolecules, 134.  pp. 354-360.  ISSN 01418130     
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065621740&doi=10.1016%2fj.ijbiomac.2019.05.044&partnerID=40&md5=51168ea44c1e3b85e3dd99c81cb71b6d
relation: 10.1016/j.ijbiomac.2019.05.044
identifier: 10.1016/j.ijbiomac.2019.05.044