TY  - JOUR
N1  - cited By 22
N2  - In this study, salting-out assisted liquidâ??liquid extraction (SALLE) as a simple and efficient extraction technique followed by high-performance liquid chromatography (HPLC) was employed for the determination of vitamin D3 in milk samples. The sample treatment is based on the use of water-miscible acetonitrile as the extractant and acetonitrile phase separation under high-salt conditions. Under the optimum conditions, acetonitrile and ammonium sulfate were used as the extraction solvent and salting-out agent, respectively. The vitamin D3 extract was separated using Hypersil ODS (250x i.d 4.6 mm, 5 µm) HPLC column that was coupled with diode array detector. Vitamin D2 was used as internal standard (IS) to offset any variations in chromatographic conditions. The vitamin D3 and the IS were eluted in 18 min. Good linearity (r2 > 0.99) was obtained within the range of 25â??600 ng gâ??1 with the limit of detection of 15 ng gâ??1 and limit of quantification of 25 ng gâ??1. The validated method was applied for the determination of vitamin D3 in milk samples. The recoveries for spiked samples were from 94.4 to 113.5. © 2019 The Authors
IS  - 8
ID  - scholars11419
TI  - Salting-out assisted liquidâ??liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples
AV  - none
JF  - Royal Society Open Science
A1  - Sazali, N.H.
A1  - Alshishani, A.
A1  - Saad, B.
A1  - Chew, K.Y.
A1  - Chong, M.M.
A1  - Miskam, M.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072216849&doi=10.1098%2frsos.190952&partnerID=40&md5=2bbb575b7250c914e1cd4adfd792401b
VL  - 6
Y1  - 2019///
PB  - Royal Society Publishing
SN  - 20545703
ER  -