%0 Journal Article
%@ 01410229
%A Chong, T.M.
%A Abdullah, M.A.
%A Fadzillah, N.M.
%A Lai, O.M.
%A Lajis, N.H.
%D 2005
%F scholars:11
%J Enzyme and Microbial Technology
%K Antioxidants; Catalysis; Cell culture; Enzymes; Oxidation; Peroxides, Elicitation; Jasmonic acid (JA); Peroxidation, Organic acids, alpha tocopherol; anthraquinone derivative; antioxidant; ascorbic acid; carotenoid; catalase; glutathione reductase; jasmonic acid, antioxidant responsive element; article; cell culture; cell growth; controlled study; lipid peroxidation; Morinda; morinda elliptica; nonhuman, Morinda
%N 4
%P 469-477
%R 10.1016/j.enzmictec.2004.11.002
%T Jasmonic acid elicitation of anthraquinones with some associated enzymic and non-enzymic antioxidant responses in Morinda elliptica
%U https://khub.utp.edu.my/scholars/11/
%V 36
%X Morinda elliptica cell cultures in an intermediary (G) medium treated with jasmonic acid (JA) elicitor, was used as a model system to understand the effects of elicitation on cell growth, anthraquinone (AQ) production, stress levels (lipid peroxidation and hydrogen peroxide (H2O2)), enzymic (catalase (CAT), ascorbate peroxidase (APO) and glutathione reductase (GR)) and non-enzymic antioxidant responses (total carotenoids, vitamin C and E). Cultures were elicitor-treated on day 12 with 50 or 100 μM JA, and harvested after 3 and 6 days of elicitation. The highest AQ content (23.6 mg g-1 DW) which was 2-fold to control but with cell growth (35 g l -1) 15 lower, was achieved with 100 μM JA. Prolonged elicitor treatment resulted in the highest level of lipid peroxidation (total MDA of 9 nmol g-1 FW) and H2O2 level (5.5 μmol g -1 FW). Total carotenoids, vitamin C and E were induced 200, 10 and 50, respectively, after 6 days of elicitation. APO though induced between 100-300, was only half the level in control, while GR was induced less than 100. CAT on the other hand was reduced 100. © 2004 Elsevier Inc. All rights reserved.
%Z cited By 47