eprintid: 10973
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/01/09/73
datestamp: 2023-11-10 03:25:33
lastmod: 2023-11-10 03:25:33
status_changed: 2023-11-10 01:14:16
type: conference_item
metadata_visibility: show
creators_name: Reddy Prasad, D.M.
creators_name: Pendyala, R.
creators_name: Senthilkumar, R.
creators_name: Azri, M.H.B.
title: Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16
ispublished: pub
keywords: Batch cell culture; Biochemistry; Biosynthesis; Carbon; Oils and fats; Rate constants; Rubber; Seed; Urea, Building blockes; Carbon and nitrogen; Experimental values; Microbial production; Poly(3-hydroxybutyrate); Rubber seed oil; Specific growth rate; Temperature range, Growth rate
note: cited By 6; Conference of 2019 International Conference on Resources and Environment Sciences, ICRES 2019 ; Conference Date: 8 August 2019 Through 10 August 2019; Conference Code:156664
abstract: Rubber seed oil is inedible plant oil that is a potential replacement for the traditional organic building blocks used in PHB biosynthesis. Herein we describe the biosynthesis of PHB by Cupriavidus necator H16 grown in media with rubber seed oil and urea serving as carbon and nitrogen sources, respectively. The method was optimized over a temperature range of 30-50 °C for batch culture studies. A maximized PHB concentration of 15.4 g/L, and dry well weight of 19.2 g/L was obtained using 20 g/L of rubber seed oil at 30°C. Experimental results were used to generate a logistic curve, and the Luedeking-Piret model was implemented to predict the specific growth rate of C. necator at 0.1898 1/h. The estimated rate constants were in good agreement with experimental values. This study demonstrated that rubber seed oil is a viable alternative to conventional carbon sources for the production of PHB using C.necator H16. © 2019 Published under licence by IOP Publishing Ltd.
date: 2019
publisher: Institute of Physics Publishing
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85078804140&doi=10.1088%2f1755-1315%2f398%2f1%2f012008&partnerID=40&md5=402f2b248bdd5851526f49aa49202b11
id_number: 10.1088/1755-1315/398/1/012008
full_text_status: none
publication: IOP Conference Series: Earth and Environmental Science
volume: 398
number: 1
refereed: TRUE
issn: 17551307
citation:   Reddy Prasad, D.M. and Pendyala, R. and Senthilkumar, R. and Azri, M.H.B.  (2019) Microbial production of poly (3-hydroxybutyrate) (PHB) from rubber seed oil using Cupriavidus necator H16.  In: UNSPECIFIED.