TY  - JOUR
JF  - Biotechnology and Bioprocess Engineering
A1  - Omar, R.
A1  - Abdullah, M.A.
A1  - Hasan, M.A.
A1  - Rosfarizan, M.
A1  - Marziah, M.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745604921&doi=10.1007%2fBF02932034&partnerID=40&md5=e95b16d263699121f3823faf57b2d985
VL  - 11
Y1  - 2006///
N2  - In this study, we have conducted kinetics and modelling studies of Centella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20 higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5 g/L, was 14 higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively. ©KSBB.
IS  - 3
ID  - scholars107
KW  - fructose; glucose; sucrose; triterpenoid
KW  -  article; bioreactor; cell culture; cell growth; cell kinetics; Centella asiatica; concentration response; dry weight; growth rate; intermethod comparison; nonhuman; nutrient uptake; pH; plant cell; plant cell culture; plant growth; plant nutrition; prediction; reactor optimization; statistical analysis; statistical model
KW  -  Centella asiatica; Fungi
EP  - 229
SN  - 12268372
N1  - cited By 17
SP  - 223
TI  - Kinetics and modelling of cell growth and substrate uptake in Centella asiatica cell culture
AV  - none
ER  -