eprintid: 10573
rev_number: 2
eprint_status: archive
userid: 1
dir: disk0/00/01/05/73
datestamp: 2023-11-09 16:37:11
lastmod: 2023-11-09 16:37:11
status_changed: 2023-11-09 16:31:43
type: article
metadata_visibility: show
creators_name: Alshishani, A.A.
creators_name: Saad, B.
creators_name: Semail, N.F.
creators_name: Mohamad Salhimi, S.
creators_name: Talib, M.K.M.
title: Salting-out assisted liquid-liquid extraction method coupled to gas chromatography for the simultaneous determination of thujones and pulegone in beverages
ispublished: pub
keywords: Beer; Beverages; Chromatography; Gas chromatography; Liquid chromatography; Liquids; Solvent extraction; Sulfur compounds, Capillary gas chromatography; Flame ionisation detector; Limit of quantifications; Liquid-liquid extraction; Pulegone; Relative standard deviations; Simultaneous determinations; Thujone, Extraction
note: cited By 9
abstract: A fast, simple and sensitive salting-out assisted liquid-liquid extraction method for the simultaneous determination of thujones and pulegone in beverage samples was developed. The extraction was achieved by adding ammonium sulphate to a miscible mixture of sample (10 mL) and acetonitrile (1.5 mL). The upper layer was directly injected into a capillary gas chromatography unit and detected using flame ionisation detector. The method was validated and calibration curve was linear with r2 > 0.99 over the range of 0.1�15 mg.kg�1 and 1�300 mg.kg�1 for thujone and pulegone, respectively. The limits of detection were 4.3 and 5.1 µg.kg�1 and the limit of quantification were 12.9 and 15.3 µg.kg�1 for thujone and pulegone, respectively. The relative standard deviation for inter- and intra -day precision was less than 10.1. The proposed method was conveniently applied for the determination of these compounds in various herbal and fruit drinks, alcoholic beer and soft drinks. The average recoveries of the analyzed compounds ranged from 97 to 110 for thujone and from 87 to 119 for pulegone in three types of beverages. Thujones were not detected in all the 43 samples analysed while pulegone was detected in two samples (< quantitation limit and 1.78 mg.kg�1). Positive identification of the thujone and pulegone peaks were provided by GC-MS. © Anas Adib Alshishani, Bahruddin Saad, Nadhiratul Farhin Semail, Salizawati Mohamad Salhimi, and Mohd Khairuddin Mohd Talib.
date: 2018
publisher: Taylor and Francis Inc.
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040979137&doi=10.1080%2f10942912.2017.1373665&partnerID=40&md5=c1ca0c64d7ce20eb60f92df1621adf62
id_number: 10.1080/10942912.2017.1373665
full_text_status: none
publication: International Journal of Food Properties
volume: 20
pagerange: S2776-S2785
refereed: TRUE
issn: 10942912
citation:   Alshishani, A.A. and Saad, B. and Semail, N.F. and Mohamad Salhimi, S. and Talib, M.K.M.  (2018) Salting-out assisted liquid-liquid extraction method coupled to gas chromatography for the simultaneous determination of thujones and pulegone in beverages.  International Journal of Food Properties, 20.  S2776-S2785.  ISSN 10942912