TY  - JOUR
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042384094&doi=10.1016%2fj.chroma.2018.02.032&partnerID=40&md5=37b588ab8ac04f0cf67e55f4a30309a4
A1  - Manaf, N.A.
A1  - Saad, B.
A1  - Mohamed, M.H.
A1  - Wilson, L.D.
A1  - Latiff, A.A.
JF  - Journal of Chromatography A
VL  - 1543
Y1  - 2018///
N2  - Sorbents were prepared by cross-linking β-cyclodextrin (β-CD) using two different types of cross-linker units at variable reactant mole ratios. The resulting polymers containing β-CD were evaluated as sorbents in micro-solid phase extraction (μ-SPE) format for the extraction of the endogenous steroids testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5α-androstane-3α,17β-diol (5αAdiol) and 5β-androstane-3α,17β-diol (5βAdiol). The best sorbent (C1; cyclodextrin polymer) showed superior extraction characteristics compared with commercial sorbents (C18 and Bond Elut Plexa). Parameters influencing the extraction efficiency of the C1 sorbent such as extraction and desorption times, desorption solvent and volume of sample were investigated. The extracts were separated using a Hypersil Gold column (50 Ã? 2.1 mm, 1.9 μm) under gradient elution coupled to a LCâ??MS/MS. The compounds were successfully separated within 8 min. The method offers good repeatability (RSD < 10) and linearity (r2 > 0.995) were within the range of 1â??200 ng mLâ??1 for T and E, 250â??4000 ng mLâ??1 for A and Etio and 25â??500 ng mLâ??1 for 5αAdiol and 5βAdiol, respectively. The method was applied for the determination of steroid profile of urine from volunteers. © 2018 Elsevier B.V.
KW  - Cyclodextrins; Desorption; Industrial chemicals; Lipids; Liquid chromatography; Mass spectrometry; Organic compounds; Phase separation; Polymers; Sorbents
KW  -  Cyclodextrin polymer; Endogenous steroids; Micro-solid-phase extractions; Molecular selectivity; Steroid profile
KW  -  Extraction
KW  -  3beta androstanediol; androstanediol; androsterone; beta cyclodextrin; epitestosterone; etiocholanolone; polymer; sorbent; steroid; testosterone; beta cyclodextrin derivative; polymer; steroid
KW  -  adult; Article; controlled study; desorption; elution; extraction; female; human; liquid chromatography-mass spectrometry; male; measurement repeatability; microsolid phase extraction; priority journal; solid phase extraction; steroid analysis; urinalysis; chemistry; isolation and purification; limit of detection; liquid chromatography; reproducibility; solid phase microextraction; tandem mass spectrometry
KW  -  beta-Cyclodextrins; Chromatography
KW  -  Liquid; Limit of Detection; Polymers; Reproducibility of Results; Solid Phase Microextraction; Steroids; Tandem Mass Spectrometry
ID  - scholars10422
EP  - 33
SN  - 00219673
PB  - Elsevier B.V.
N1  - cited By 19
SP  - 23
TI  - Cyclodextrin based polymer sorbents for micro-solid phase extraction followed by liquid chromatography tandem mass spectrometry in determination of endogenous steroids
AV  - none
ER  -