@article{scholars10407, note = {cited By 17}, journal = {Biocatalysis and Agricultural Biotechnology}, year = {2018}, doi = {10.1016/j.bcab.2018.01.011}, title = {Effects of macro/micronutrients on green and brown microalgal cell growth and fatty acids in photobioreactor and open-tank systems}, pages = {10--17}, publisher = {Elsevier Ltd}, volume = {14}, author = {Usman Shah, S. M. and Abdullah, M. A.}, abstract = {This study described the optimization of growth parameters that affected locally-isolated green Nannochloropsis oculata and Tetraselmis suecica and brown Isochrysis galbana and Pavlova lutheri microalgae using Response Surface Methodology (RSM). Under deficiency conditions of 10{\^a}??65 g L{\^a}??1 KNO3, 3{\^a}??7.5 g L{\^a}??1 Na2HPO4 and 2.5 g L{\^a}??1 FeCl3 the highest lipid accumulation of 37.3, 23.6, 28.3 and 37.2, with slightly reduced cell growth of 0.64, 0.49, 0.54 and 0.38 g L{\^a}??1 were achieved for N. oculata, T. suecica, I. galbana and P. lutheri, respectively. The macronutrients significantly influenced the biomass and lipid content positively. However, the interaction of phosphate-phosphate for N. oculata, and nitrate-nitrate for I. galbana may affect cell growth negatively. The highest biomass of 0.62{\^a}??0.96 g L{\^a}??1 and lipid content of 31.6{\^a}??42.2 in 5 L PBR and the highest biomass of 0.45{\^a}??0.72 g L{\^a}??1 and lipid content of 24.4{\^a}??38.5 in 300 L open tank were achieved for all the four species. The total saturated fatty acids (44.3{\^a}??63.8 and 30.4{\^a}??55.03); monounsaturated fatty acids (6.1{\^a}??37.0 and 4.2{\^a}??13.1); and polyunsaturated fatty acids (8.3{\^a}??22.3 and 1.02{\^a}??15.2) were obtained, respectively, with pentadecanoic (C15:0), palmitic (C16:0), palmitoleic (C16:1), heptadecanoic (C17:0), oleic (C18:1), eicosanoic (C20:0), eicosapentaenoic (C20:5) and docosahexaenoic (C22:6) as predominant fatty acids. {\^A}{\copyright} 2018 Elsevier Ltd}, issn = {18788181}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041628373&doi=10.1016\%2fj.bcab.2018.01.011&partnerID=40&md5=561143e1ba09915dd3d0dee52f462f6b} }