TY - JOUR N2 - To determine the influence of nutrients on the rate of biodegradation, a five-level, three-factor central composite design (CCD) was employed for bioremediation of seawater artificially contaminated with crude oil. Removal of total petroleum hydrocarbons (TPH) was the dependent variable. Samples were extracted and analyzed according to US-EPA protocols. A significant (R2=0.9645, P<0.0001) quadratic polynomial mathematical model was generated. Removal from samples not subjected to optimization and removal by natural attenuation were 53.3 and 22.6, respectively. Numerical optimization was carried out based on desirability functions for maximum TPH removal. For an initial crude oil concentration of 1g/L supplemented with 190.21mg/L nitrogen and 12.71mg/L phosphorus, the Design-Expert® software predicted 60.9 hydrocarbon removal; 58.6 removal was observed in a 28-day experiment. © 2010 Elsevier Ltd. IS - 24 ID - scholars1018 KW - Bio-augmentation; Central composite designs; Dependent variables; Design-expert; Desirability function; Hydrocarbon removal; Natural attenuation; Numerical optimizations; Oil concentration; Optimal conditions; Petroleum; Quadratic polynomial; Response Surface Methodology; Total petroleum hydrocarbons KW - Bioremediation; Biotechnology; Crude oil; Degradation; Environmental Protection Agency; Extraction; Hydrocarbons; Mathematical models; Microbiology; Nitrogen removal; Oil spills; Optimization; Petroleum chemistry; Phosphorus; Pollution; Seawater; Surface properties KW - Biodegradation KW - petroleum; petroleum derivative; phosphorus; sea water KW - biodegradation; bioremediation; crude oil; experimental study; nitrogen; numerical model; nutrient availability; oil spill; petroleum hydrocarbon; phosphorus; seawater; software KW - article; attenuation; biodegradation; bioremediation; controlled study; mathematical model; observation; priority journal; statistical significance; waste component removal; water contamination; water management KW - Analysis of Variance; Biodegradation KW - Environmental; Environmental Remediation; Models KW - Chemical; Nitrogen; Petroleum; Phosphorus; Seawater; Time Factors A1 - Zahed, M.A. A1 - Aziz, H.A. A1 - Isa, M.H. A1 - Mohajeri, L. A1 - Mohajeri, S. JF - Bioresource Technology UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-77956180922&doi=10.1016%2fj.biortech.2010.07.077&partnerID=40&md5=bbf48bccae48fe2e58d1a3ca56c49c26 VL - 101 Y1 - 2010/// N1 - cited By 97 TI - Optimal conditions for bioremediation of oily seawater SP - 9455 AV - none EP - 9460 SN - 09608524 ER -