%0 Journal Article %@ 09608524 %A Zahed, M.A. %A Aziz, H.A. %A Isa, M.H. %A Mohajeri, L. %A Mohajeri, S. %D 2010 %F scholars:1018 %J Bioresource Technology %K Bio-augmentation; Central composite designs; Dependent variables; Design-expert; Desirability function; Hydrocarbon removal; Natural attenuation; Numerical optimizations; Oil concentration; Optimal conditions; Petroleum; Quadratic polynomial; Response Surface Methodology; Total petroleum hydrocarbons, Bioremediation; Biotechnology; Crude oil; Degradation; Environmental Protection Agency; Extraction; Hydrocarbons; Mathematical models; Microbiology; Nitrogen removal; Oil spills; Optimization; Petroleum chemistry; Phosphorus; Pollution; Seawater; Surface properties, Biodegradation, petroleum; petroleum derivative; phosphorus; sea water, biodegradation; bioremediation; crude oil; experimental study; nitrogen; numerical model; nutrient availability; oil spill; petroleum hydrocarbon; phosphorus; seawater; software, article; attenuation; biodegradation; bioremediation; controlled study; mathematical model; observation; priority journal; statistical significance; waste component removal; water contamination; water management, Analysis of Variance; Biodegradation, Environmental; Environmental Remediation; Models, Chemical; Nitrogen; Petroleum; Phosphorus; Seawater; Time Factors %N 24 %P 9455-9460 %R 10.1016/j.biortech.2010.07.077 %T Optimal conditions for bioremediation of oily seawater %U https://khub.utp.edu.my/scholars/1018/ %V 101 %X To determine the influence of nutrients on the rate of biodegradation, a five-level, three-factor central composite design (CCD) was employed for bioremediation of seawater artificially contaminated with crude oil. Removal of total petroleum hydrocarbons (TPH) was the dependent variable. Samples were extracted and analyzed according to US-EPA protocols. A significant (R2=0.9645, P<0.0001) quadratic polynomial mathematical model was generated. Removal from samples not subjected to optimization and removal by natural attenuation were 53.3 and 22.6, respectively. Numerical optimization was carried out based on desirability functions for maximum TPH removal. For an initial crude oil concentration of 1g/L supplemented with 190.21mg/L nitrogen and 12.71mg/L phosphorus, the Design-Expert® software predicted 60.9 hydrocarbon removal; 58.6 removal was observed in a 28-day experiment. © 2010 Elsevier Ltd. %Z cited By 97