TY  - JOUR
Y1  - 2018///
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048716262&doi=10.1016%2fj.ijbiomac.2018.05.084&partnerID=40&md5=54595d2b100028262bdbd41535d60001
JF  - International Journal of Biological Macromolecules
A1  - Ong, C.C.
A1  - Gopinath, S.C.B.
A1  - Rebecca, L.W.X.
A1  - Perumal, V.
A1  - Lakshmipriya, T.
A1  - Saheed, M.S.M.
VL  - 116
N2  - There are different clotting factors present in blood, carries the clotting cascade and excessive bleeding may cause a deficiency in the clotting Diagnosis of this deficiency in clotting drastically reduces the potential fatality. For enabling a sensor to detect the clotting factors, suitable probes such as antibody and aptamer have been used to capture these targets on the sensing surface. Two major clotting factors were widely studied for the diagnosis of clotting deficiency, which includes factor IX and thrombin. In addition, factor IX is considered as the substitute for heparin and the prothrombotic associated with the increased thrombin generation are taking into account their prevalence. The biosensors, surface plasmon resonance, evanescent-field-coupled waveguide-mode sensor, metal-enhanced PicoGreen fluorescence and electrochemical aptasensor were well-documented and improvements have been made for high-performance sensing. We overviewed detecting factor IX and thrombin using these biosensors, for the potential application in medical diagnosis. © 2018 Elsevier B.V.
KW  - antibody; aptamer; blood clotting factor 9; bovine serum albumin; ethanolamine; gold nanoparticle; macrogol; thrombin; blood clotting factor 9; thrombin
KW  -  antigen binding; blood clotting factor deficiency; conformational transition; electrochemical detection; evanescent field coupled waveguide mode sensor; hemophilia A; hemophilia B; human; impedance spectroscopy; partial thromboplastin time; Review; spectrofluorometry; surface plasmon resonance; animal; blood; blood clotting disorder; chemistry; metabolism; procedures
KW  -  Animals; Aptamers
KW  -  Nucleotide; Blood Coagulation Disorders; Factor IX; Humans; Surface Plasmon Resonance; Thrombin
ID  - scholars10052
SN  - 01418130
PB  - Elsevier B.V.
EP  - 773
AV  - none
N1  - cited By 32
SP  - 765
TI  - Diagnosing human blood clotting deficiency
ER  -