Introduction: Emulsification of produced immiscible mixed liquid of oil and water is a problem frequently recognized in surface production facilities in oil fields. The formed emulsions are required to be demulsified to avoid the negative consequences on piping and processing equipment. Nowadays, chemical demulsification is a preferable method to avoid or retard emulsification during the process of oil treatment. Materials and Methods: In the present study, Polyvinylpyrrolidone, poly-1-(2-oxo-1-pyrrolidinyl)-ethylene has been experimentally investigated as a chemical demulsifier for water-in-oil emulsion formulated by mixing a crude oil with synthesized oilfield produced water. Oilfield brines were synthesized by dissolving NaCl in deionized water to obtain salinity similar to the oilfield produced water. The sample of water-oil emulsion was prepared by agitation process at the condition of controlled rpm speed, ambient temperature and concentration of emulsifying agent. Stability of the emulsion was investigated using Turbiscan MA 2000 instrument. Results: The efficiency of the demulsification was screened using Bottle Test. It has been found that Polyvinylpyrrolidone, poly-1-(2-oxo-1- pyrrolidinyl)-ethylene accelerates the separation of the emulsion as compared to blank ones (with no addition of PVP). A maximum demulsification performance of the PVP was observed at an optimum concentration of 60 ppm. © 2017 Mohyaldinn et al.