Physical and chemical properties of biodiesel are influenced by the structural features of fatty acid, such as with saturated, monounsaturated and polyunsaturated fatty acids. In this study, seven non-edible oils have been selected, which include waste cooking oil derived palm olein, Calophyllum inophyllum, jatropha oil, castor oil, rubber seed oil, kapok seed oil and karanja oil. The critical parameters, e.g. cetane number (CN), iodine value (IV) and oxidation stability (OS) of biodiesel were correlated with the degree of unsaturated (DU) fatty acid, whereas the cold filter plugging point (CFPP) was correlated with the long chain saturated factor (LCSF). To meet the minimum EU requirement of EN 14214 of the critical parameter, the DU value of the CN was �133.5, IV �123.2 and OS �98.9. The LCSF values satisfied the Spanish regional standard - RD 61/2006 in summer (0°C) at �8.4 and winter (-10°C) at �0.1 of the CFPP. Based on the composition of the saturated, monounsaturated and polyunsaturated fatty acids, a triangular chart for the biodiesel property prediction was developed. This can then be used as a reference for non-edible oils. © 2015 Springer-Verlag Berlin Heidelberg.