Exhaled breath (EB) contains thousands of volatile and nonvolatile biomolecules. EB analysis is non-invasive and convenient to patients than blood or urine tests. The exhaled biomolecules have long been studied and recognized to have some potential biomarkers for diagnosis of diseases, evaluation of metabolic disorders and monitoring drug efficiency. For instance, Biomarkers such as exhaled hydrogen peroxide (H2O2) and exhaled nitric oxide are associated with inflammatory lung diseases, ammonia is used as a biomarker for kidney diseases and exhaled acetone is related to glucose concentration in blood and so it is used for diabetes diagnosis. H2O2 concentration in EB increases with the severity of lung diseases such as asthma, chronic obstructive pulmonary disease (COPD), and adult respiratory distress syndrome (ARDS). Different methods are used to measure H2O2 in exhaled breath condensate (EBC). In EBC the EB is collected in a condensate unit and then H2O2 concentration in the collected sample is detected using titrimetric, spectrophotometry, fluorescence, chemiluminescence and electrochemical sensors. Recently, some works have been done to measure the concentration of H2O2 in its vapor phase without a need for condensation units. The aim of this paper is to review and summarize the current methods being used to measure the concentration of H2O2 in EB to identify inflammatory lung diseases, and to discuss the advantages and disadvantages of these methods. © (2015) Trans Tech Publications, Switzerland.