Microalgae have been vastly investigated throughout the world for possible replacement of fossil fuels, besides utilization in remediation of leachate, disposal of hypersaline effluent and also as feedstock for marine organisms. This research particularly has focused on locally available marine microalgae sample and Nannochloropsis oculata for potential mass production of microalgae biomass. Biomass produced by sample 1 and sample 2 is 0.6200 g/L and 0.6450 g/L respectively. Meanwhile, sample 3 and N. oculata has obtained maximum biomass concentration of 0.4917 g/L and 0.5183 g/L respectively. This shows that sample 1 and sample 2 has produced approximately 20 higher biomass concentration in comparison to sample 3 and N. oculata. Although sample 3 and N. oculata is slightly lower than other samples, the maximum biomass was achieved four days earlier. Hence, the specific growth rate of sample 3 and N. oculata is higher; meanwhile the specific growth rate of N. oculata is the highest. Optical density measurements of all the sample throughout the cultivation period also correlates well with the biomass concentration of microalgae. Therefore, N. oculata is finally selected for utilization in mass production of microalgae biomass. © Published under licence by IOP Publishing Ltd.