Spectral Library is data archive of spectral signatures of various natural and man-made materials. In oil and gas industry, spectral library might not be heard often, but these tools can pose a great help for future oil and gas exploration. A developing spectral library for hydrocarbon is basically a new advancement in this field, and this project may implement the spectral library on global hydrocarbon seeps in the future. In this paper, the procedure in the developing spectral library from vegetation stress was demonstrated. In order to obtain these spectral signatures of hydrocarbon, the usage of hyperspectral remote sensing analysis and spectroradiometer is required. But for the early stage of development, spectrophotometer with the range up to 740 ηm was used to extract the spectral signature of the plants in term of green percentage. Nine samples of palm oil trees represent the stressed vegetation was planted with 10 and 40 porosity of crude oil and control samples having three palm oil trees for each of those. The vegetation has been left in an open environment with enough sunlight and watered daily. The significance result of changes in green percentage in the spectral signature of the trees with different porosity level was compared to the control samples. It shows that the existence of crude oil influences the health of the vegetation which has been notified through the spectral signature of the plant. This paper signifies a first step towards the development of spectral signature of hydrocarbon for oil and gas exploration. © (2014) Trans Tech Publications, Switzerland.