Conventional and non-conventional Surfactants were utilized to fabricate com-starch nanoparticles. The surfactants used were pluronic F-68, span 20, Aerosol TR and Aerosol OT. Starch, in premix with either of the surfactants with concentration 0.5 to 2 were grinded in Netsch MiniCer® laboratory circulation machine (Netsch, Germany) at 25 Hz. Zicronium beads of 1mm diameter were used as the grinding media. At 0.5 hr grinding time, 0.5 pluronic reduced corn starch to the lowest size of 335 ± 5.57 nm. The other surfactants yielded particle size equal to or above 382 ±9.5 nm. However, with 1 hr grinding, 1 pluronic F-68 reduced the particle size of corn starch to the minimum level of 353 ±14.50 nm. At 1 hr grinding, the other surfactants reduced the size equal to or above 399 ± 9.50 nm. The lowest PDI was 0.393 ± 0.06 and 0.417 ± 0.02, respectively with 1 TR-OT at 0.5 hr and 2 TR-OT at 1 hr grinding. Pluronic demonstrated the highest efficiency in size reduction followed by ATR-AOT (1:4).