A biophotovoltaic (BPV) device is a type of bioelectrochemical cell that shows potential for renewable energy production using photosynthetic exoelectrogenic microorganisms. However, the technology currently has limitations due to its relatively low power output and high dependence on biofilm attachment. Thus, this paper examines the effect of hydrodynamic aeration on the bioelectricity generation of BPV devices. Aeration is widely applied in microalgae cultivation to improve the mixing effect between microalgae cells, culture medium, and air bubbles. The methodology involves the cultivation of Spirulina platensis, device fabrication, voltage measurement in both non-aeration and aeration modes, and the use of polarization curves to determine the device performance under a gradually rising external load. The BPV devices are affected by abnormal voltage drop under high external loads, which is called power overshoot. The use of aeration successfully solved the power overshoot issues over time, but it also resulted in a gradual reduction in the voltage output and power density of the BPV devices. During aeration, the BPV devices produced a peak power density of 0.121 ± 0.056 mWm�2 on Day 1, which declined to 0.048 ± 0.025 mWm�2 on Day 13. The blowing of air bubbles had some side effects on BPV devices, including causing the attachment of Spirulina cells to the container walls and accelerating the evaporation of the culture medium. This study emphasizes the role of hydrodynamic aeration in the bioelectricity generation process and opens up possibilities for wider application of BPV devices in renewable energy production. © 2023 Elsevier B.V.