The Bakun-Murum (BM) catchment region of the Rajang River Basin (RRB), Sarawak, Malaysia, has been under severe threat for the last few years due to urbanization, global warming, and climate change. The present study aimed to evaluate the time series analysis and impact assessment of the temperature changes on the vegetation/agricultural lands and the water availability within the BM region. For this purpose, the Landsat data for the past thirty years (1990�2020) were used. Remote sensing techniques for estimating the surface temperatures and variation within the vegetation and water bodies were utilized, and validation was done using on-ground weather stations. Google Earth Engine (GEE) and other RS & GIS tools were used for analyzing the time series trends of land surface temperature (LST), normalized difference vegetation index (NDVI), and normalized difference water index (NDWI). The results exposed an overall rise of 1.06 °C in the annual mean temperatures over the last thirty years. A maximum annual mean NDVI of 0.48 was recorded for 2018 and 2019. The lowest annual mean NDVI (0.27) was observed in 2005. The annual mean NDWI increased to 0.48 in 2018 and 2019, respectively. The statistical correlation results revealed the coefficient of determination (R2) of 0.09 and 0.13 for the annual mean LST and annual mean NDVI and the annual mean LST and annual mean NDWI, respectively. Moreover, the Mann-Kendall trend test for the annual mean temperature series indicates a slightly increasing trend with Sen's slope of 0.03 °C/year. It is found that there is a positive trend in the annual mean rainfall patterns, as Sen's slope indicates a yearly increase of 50.58 mm/year. This study found significant changes in the LST, NDVI, and NDWI of the BM catchment region during the last thirty years, demanding the concerned authorities' instant attention to alleviate the adverse effects of such changes to protect the ecosystem. © 2022 Elsevier B.V.