Biodiesel production from waste such as wastewater sludge, palm oil mill effluent and dairy waste provide more sustainable approach and greener energy. This paper analyzes the kinetic modelling for biodiesel production from dairy waste scum oil via microwave heating transesterification. The results from this study show that transesterification of dairy waste scum oil is a first order kinetic which defines that the biodiesel production is dependent on the concentration of one reactant linearly. The activation energy for the reaction was validated to be 18.12 J/mol and frequency factor was 3.163 s-1. It is validated that the activation energy of transesterification process in this study is lower than the past works have been done. The comparison of the value is as tabulated in the kinetic analysis section. © 2023, Association of American Publishers. All rights reserved.