The shelf-life of catalyst ink for fabricating polymer electrolyte fuel cells (PEFCs) is relevant for large-scale manufacturing with unforeseen production stops. In this study, the storage effects on the physicochemical characteristics of catalyst ink (Pt/C, Nafion, 2-propanol, water) and subsequently manufactured catalyst layers are investigated. Sedimentation analysis showed that catalyst particles are not fully stabilized by charge interaction induced by Nafion. Acetone was found to be an oxidation product, even in freshly prepared ink with platinum catalyzing the reaction. Rotating disk electrode analysis revealed that the electrochemically active surface area is, overall, minimally increased by storage, and the selectivity towards water formation (4-electron pathway) is unharmed within the first 48 h of storage. MEAs prepared from stored ink reach almost the same current density level after conditioning via potential cycling. The open-circuit voltage (OCV) increases due to increased catalyst availability. Scanning electron microscopy and mercury intrusion porosimetry showed that with increasing acetone content, the pore structure becomes finer, with a higher specific surface area. Electrochemical impedance spectroscopy revealed that this results in a more hindered mass transfer but lowered charge transfer resistance. The MEA with the highest OCV and power output and the lowest overall cell resistance was fabricated from catalyst ink stored for a duration of four weeks. © 2023 by the authors.