Advanced oxidation process (AOPs) are technologies based on the intermediacy of hycroxyl radical and other radicals to oxidize recalcitrant, toxic nad no biodegradable cpmpounds to various by-products. In this paper, Oxytetracycline hydrochloride (OTC) was degraded inside a UV-H 2O 2 system which utilize hydroxyl radical generated from H 2O 2 to attack OTC. Concentration of H 2O 2, temperature and pH was varied in this experiment. To monitor OTC degradation, TOC and UV-VIS spectra analysis were carried out. Constant OTC concentration at 250 ppm was used. About 93.06 of TOC removal was achieved at 40C and 1 eq mole ratio H 2O 2 to OTC. After 180 min irradiation, UV-Vis spectra of OTC have shown total disappearance of OTC peak. HPLC analyses has shown about 93.08 of OTC were degraded after 180 min irradiated inside UV-H 2O 2 system. © 2011 IEEE.