Agriculture materials of oil palm tree and forestry such as rubber tree are commonly utilised and this can be contributed to the wastes. Therefore, this research is looking for value-added to these types of materials from the production of pyrolysis oil. This paper was intended to determine the best ratio of feedstock to produce high quantity of bio-oil yield and to determine properties of chemicals produced in bio-oil. The samples of empty fruit bunches (EFB) with palm kernel shell (PKS), palm leaves (PL) and sawdust (SDT) were co-pyrolyzed with different ratios (25:75, 50:50 and 75:25) to produce liquid oil, and the yield was analyzed and characterized by using GC-MS. The results show the mixture of EFB:PKS with ratio of 25:75 produced the highest yield of 40.53, meanwhile, the co-pyrolysis of EFB:SDT produced the lowest yield which was 19.25. The chemicals for each bio-oil were determined and the major organic chemicals such as phenols, aldehydes group, methyl alcohol and acetic acids were found for the purpose products of our fine chemicals. © 2021, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.