The aims of this study were to optimize the supercritical carbon dioxide extraction conditions on the extraction yield and hydrolyzable tannins content of Quercus infectoria (QI) galls and determine the bioactivities of the gall extracts related to wound healing. Experimentally, the QI gall was extracted using the supercritical carbon dioxide (SC-CO2) extraction method at different operating conditions which were pressure (20 to 30 MPa), temperature (50 to 70°C), and mean particle size (0.50 to 1.50 mm). The optimum values of pressure, temperature and mean particle size to achieve optimum responses (galls extraction yield: 6.02, tannic acid concentration: 6,149.71 mg/g sample; gallic acid concentration: 96.85 mg/g sample) were 28.11 MPa, 50.43°C, and 1.25 mm, respectively. The findings also demonstrated that the extracts possess antioxidant activity and cell proliferation and migration abilities. Novelty Impact Statement: We declare that the work submitted for publication is original, previously unpublished in English or any other language(s), and not under consideration for publication elsewhere. I also certify that all the authors have approved the paper for release and are in agreement with its content. Furthermore, there are no conflict interest in this study. Thank you for your consideration of this manuscript. © 2020 Wiley Periodicals LLC.