The complexity in aircraft ground handling operations has led this study to conduct a holistic assessment on the ground handling safety culture, with the purpose of preventing accidents at the workplace. This study adopts the multiple perspective assessment model that allows the integration of two mixed methods designed with data triangulation. The qualitative results generated seven themes and eight themes respectively, in which were later generalized and developed for quantitative instrument. The quantitative results showed the industry's safety culture performance and its influence on the safety performance outcome constructs. This study is expected to contribute towards the development of safety culture evaluation for this industry and becomes a stakeholder's indicator to improve safety and health standards. © 2021 Elsevier Ltd