Nowadays, Copper, Cu(II) is one of the toxic heavy metals that has been discharged increasingly to the water resources and cause serious considerations due to their irresolvable and harmful properties. Generally, the electrospun membrane fabricated through electrospinning techniques can perform efficient microfiltration capability which can effectively remove Cu(II) in aqueous solution while Deep Eutectic Solvents (DES) formed between choline hydroxide and amino acid can be further enhance the performance as an extractant in the separation of Cu(II) ions. A comparative study was done from the perspective of sigma profile, sigma potential and activity coefficient at infinite dilution of heavy metal ions (Fe3+, Ni2+, Cu2+, and Pb2+) using COSMO-RS and proposed research experiments were on characterization and performance of electrospun Nylon 6,6 NFM and with five different molar ratios between choline hydroxide and glycine as AAILs with 1:1, 1:2, 1:3, 1:4, and 1:5 for copper removal. The solvent capacity value at infinite dilution of the amino anion and choline cation is 2 which indicate that all these combinations could be used for heavy metal removal purpose. Experimental results showed that Nylon 6,6/DES with 1:5 M ratio between choline hydroxide and glycine gives the highest hydrophilicity and largest membrane diameter. Moreover, Nylon 6,6/DES with 1:2 M ratio between choline hydroxide and glycine gives the highest permeability (5504 L/m2.hr.bar) and Cu(II) rejection (80) until reached steady state. The Cu (II) metal ions adsorption equilibrium on the membrane was 295.66 mg/g after 24 h. © 2021 Elsevier B.V.