The heat transfer process takes place in numerous applications through the natural convection of fluids. Investigations of the natural convection heat transfer in enclosures have gained vital importance in the last decade for the improvement in thermal performance and design of the heating/cooling systems. Aspect ratios (AR=height/length) of the enclosures are one of the crucial factors during the natural convection heat transfer process. The investigated fluids consisting of air, water, engine oil, mercury, and glycerine have numerous engineering applications. Heat transfer and fluid flow characteristics are studied in 3-dimensional rectangular enclosures with varying aspect ratios (0.125 to 150) using computational fluid dynamics (CFD) simulations. Studies are carried out using the five different fluids having Prandtl number range 0.01 to 4500 in rectangular enclosures with the hot and cold surface with varying temperature difference 20K to 100K. The Nusselt number and heat transfer coefficients are estimated at all conditions to understand the dependency of ARs on the heat transfer performance of selected fluids. Temperature and velocity profiles are compared to study the flow pattern of different fluids during natural convection. The Nusselt number correlations are developed in terms of aspect ratio and Rayleigh number to signify the natural convection heat transfer performance. © The Authors, published by EDP Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/)