Ionic liquids are promising materials for several applications. Amino acid based ionic liquids (AAILs) as a sort of a new generation of ionic liquids are recently proposed taking advantage of hydrogen bonding group and low toxicity. Three types of AAILs are examined in this work; namely 1-Octyl-3-Methylimidazolium Glycine OMIMGly, 1-Octyl-3-Methylimidazolium Alanine OMIMAla and 1-Octyl-3-Methylimidazolium Proline OMIMPro. The pH and molar conductivity are measured at ambient conditions for AAILs aqueous solutions at different concentrations; specifically 1, 10, 20, and 30 wt.%. The experimental results show that all AAILs solutions are alkaline (pH>7) where OMIMPro has the highest pH compared to its counterparts. The trend is reversed for molar conductivity as OMIMGly showed the highest molar conductivity. These results are attributed to the smaller size of Gly compared to Pro and Ala. However, adding AAILs increases the molar conductivity of the water. © The Authors, published by EDP Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/)