The comprehensive studies have been accomplished to examine the effect of Ni loading over H-Y zeolite for hydrodeoxygenation (HDO) of crude rubber seed oil. Monometallic Ni/H-Y zeolite catalysts were synthesized via wet impregnation method with different wt. of Ni i.e. (3, 12 and 15 wt. Ni) doped on H-Y zeolite. Field Emission Scanning Electron Microscopy (FESEM) coupled with Electron Dispersive X-ray analysis (EDX), X-ray Diffraction (XRD), Brunauer�Emmett�Teller (BET) Surface Area and Temperature Programmed Reduction (H2TPR) techniques were used to study the textural properties of the hydrocracking catalysts. The physiochemical characterization of synthesized catalysts revealed that the metal oxides were homogenously dispersed over the support with higher surface area and low reduction temperature. The hydrodeoxygenation of rubber seed oil was performed in fixed bed tubular reactor at 350°C, 35 bar H2 pressure, H2: Oil ratio 1000 N (cm3/cm3), and weight hour space velocity (WHSV) =1 h-1. The liquid products analysis revealed that the diesel range hydrocarbons (C15-C18) yield increased with increasing Ni loading. 15 wt. Ni/H-Y zeolite showed comparatively higher yield of diesel range hydrocarbons i.e.(30 wt.) among all the synthesized catalysts. All the catalysts showed >99 triglycerides conversion into diesel range hydrocarbons and other oxygenated intermediates. The selectivity for diesel range hydrocarbons was obtained as follows C15 (14.5 wt.), C16 (6.8 wt.), C17 (10.4 wt.) and C18 (2.4 wt.) for 15 wt. Ni/H-Y zeolite catalyst. Mostly, all catalysts produced lower alkanes due to high acidity and hydrocracking property of H-Y zeolite. The HDO activity showed that H-Y zeolite catalyst is more favorable for hydrocracking of crude vegetable oil to produce lower alkanes, compared to diesel range hydrocarbons due to its high acidity. © 2019 Elsevier Ltd. All rights reserved.