Stroop color-word test (STW) is a reliable method that has been used in laboratory to induce stress to the subject. This paper describes the development of Stroop color-word Test which was implemented based on five sections of test condition: i. Introductory Section (IS), ii. Resting Section (RS), iii. Congruent Section (CS), iv. Incongruent Section 1 (ICS-1), and v. Incongruent Section 2 (ICS-2). This test condition was identified from normal state until the highest stress state. Psychology experiment and MATLAB software were used in implementing the stress test. Electroencephalogram (EEG) signal of six healthy subjects was recorded while conducting this test. Power Spectral Density (PSD) was computed and analyzed. Results found that PSD can differentiate the level of stress from normal until the highest stress state. As such, it can be used as computer-assisted tool for stress detection. © 2019 Elsevier Ltd. All rights reserved.