The lignocellulose biomass resource can be used not only as direct energy in combustion, but also as a more valuable fuel after conversion and upgrading process. Fast pyrolysis is an approach that produces bio-oil from biomass. However, it is very difficult to directly utilize the pyrolysis oil because of the presence of oxygenated compounds (e.g., acids, esters, alcohols, ketones, furans and phenols). Hydrodeoxygenation (HDO) is a prominent process to upgrade bio-oil. The HDO experimental and kinetic study were conducted using PdFe/Al-MCM-41 catalyst in a tubular fixed-bed reactor. Kinetic model was proposed and applied to 21 reaction pathways, 13 compounds. The detailed reaction pathways of HDO of guaiacol and their reaction rates were clarified for a PdFe catalyst. The major pathway of HDO of guaiacol is direct production of phenol and then benzene. The detailed kinetic model is an effective tool to estimate the major reaction pathways of HDO process. ©BEIESP.