In this study, the microclimate of the test room was regulated using thermoelectric air duct cooling system (TE-AD) operated at input powers-240 W, 360 W, 480 W, 600 W, 720 W, and 840 W, on subsequent nights. Fifteen (15) healthy male volunteers were recruited to sleep under these test conditions and their sleep quality was assessed by studying objective measures such as sleep onset latency (SOL), mean skin temperature and heart rate as well as subjective parameters like predicted mean vote (PMV) and predicted percentage of dissatisfied (PPD). There was a consistent improvement on all studied parameters when the power of the system was increased from 240 W to 720 W. The mean sleep onset latency time was reduced from (M = 40.7 +/� 0.98 min) to (M = 18.33 +/� 1.18 min) when the operating power was increased from 240 W to 720 W, denoting an improvement in sleep quality. However, increasing the power further to 840 W resulted in deteriorating cooling performance of the TE-AD system leading to an increase in temperature of the test room and reduction in sleep comfort. Analysis of subjective indices of thermal comfort viz. PMV and PPD revealed that subjects are highly sensitive towards variations in microclimate achieved by changing the operating power of the TE-AD. This device was also found to be environmentally sustainable, with estimated reduction in CO2 emission calculated to be around 38 as compared to the conventional air-conditioning. © 2019 by the authors.